Growth and recombinant protein expression with Escherichia coli in different batch cultivation media

@article{Hortsch2010GrowthAR,
  title={Growth and recombinant protein expression with Escherichia coli in different batch cultivation media},
  author={Ralf Hortsch and Dirk Weuster-Botz},
  journal={Applied Microbiology and Biotechnology},
  year={2010},
  volume={90},
  pages={69-76}
}
Parallel operated milliliter-scale stirred tank bioreactors were applied for recombinant protein expression studies in simple batch experiments without pH titration. An enzymatic glucose release system (EnBase), a complex medium, and the frequently used LB and TB media were compared with regard to growth of Escherichia coli and recombinant protein expression (alcohol dehydrogenase (ADH) from Lactobacillus brevis and formate dehydrogenase (FDH) from Candida boidinii). Dissolved oxygen and pH… CONTINUE READING

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Weuster-Botz D (2010a) Power consumption and maximum energy dissipation in a milliliter-scale bioreactor

R Hortsch
2010

Whole-cell biocatalysis: evaluation of new hydrophobic ionic liquids for efficient asymmetric reduction of prochiral ketones

S Bräutigam, D Dennewald, +3 authors D Weuster-Botz
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