Growth and maintenance of an embryogenic cell culture of daylily (Hemerocallis) on hormone-free medium.

@article{Smith1991GrowthAM,
  title={Growth and maintenance of an embryogenic cell culture of daylily (Hemerocallis) on hormone-free medium.},
  author={David L. Smith and Abraham D. Krikorian},
  journal={Annals of botany},
  year={1991},
  volume={67},
  pages={443-9}
}
Callus cultures of the diploid daylily (Hemerocallis) clone Autumn Blaze' were initiated and maintained in hormone-containing nutrient medium. At various times (from 6 weeks to 1 year) after being initiated, hormone-derived cultures were evaluated for their ability to be maintained and to multiply on hormone-free medium at low pH (between pH 4 and 4.5). Cultures had to be exposed to hormone-containing medium for at least 12 weeks before they could be maintained on hormone-free medium at low pH… CONTINUE READING
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