Growth and enrichment medium for detection and isolation of Shiga toxin-producing Escherichia coli in cattle feces.

@article{Hussein2008GrowthAE,
  title={Growth and enrichment medium for detection and isolation of Shiga toxin-producing Escherichia coli in cattle feces.},
  author={Hussein Salih Hussein and Laurie M. Bollinger and Mark Hall},
  journal={Journal of food protection},
  year={2008},
  volume={71 5},
  pages={
          927-33
        }
}
Detection methods of Shiga toxin-producing Escherichia coli (STEC) in cattle feces varied in using enrichment media containing different antibiotic combinations. To examine efficacy of a new detection method for STEC, three O157:H7 (ATCC 43889, 43890, and 43895) and 41 non-O157:H7 (members of the O1, O15, O26, O86, O103, O111, O125, O127, O128, O136, O146, O153, O158, O165, O166, and O169 serogroups) isolates were tested. These isolates were grown in tryptic soy broth for 6 h, and their… 
Comparison of Agar Media for Detection and Quantification of Shiga Toxin-Producing Escherichia coli in Cattle Feces.
TLDR
It is suggested that CHROMagar STEC performs better than Possé or SHIBAM for detection of STEC in bovine feces, but adjustments in the concentrations of novobiocin and potassium tellurite in the latter two media result in significant improvements in their performance.
Detection methods and intestinal adherence of non-O157 shiga toxin-producing Escherichia coli
Detection of Shiga toxin-producing Escherichia coli (STEC) in complex sample matrices remains challenging. In an attempt to improve detection, nonselective and selective enrichment broths were
Comparison of Enrichment Broths for Supporting Growth of Shiga Toxin-Producing Escherichia coli
TLDR
Overall, STEC grew significantly better when enriched in EC compared to TSB, and modification of TSB by the addition of bile salts improved the growth and detection of STEC compared toTSB alone.
Effect of the enrichment time and immunomagnetic separation on the detection of Shiga toxin-producing Escherichia coli O26, O103, O111, O145 and sorbitol positive O157 from artificially inoculated cattle faeces.
TLDR
Recovery of serotype O157 (s- and s+) was significantly improved, whereas for O26, O103, O111, and O145 no significant effect was observed, and the protocol can be simplified if only certain serotypes have to be investigated.
Isolation and characterization of shiga toxin-producing escherichia coli serogroups O26, O45, O103, O111, O113, O121, O145, and O157 shed from range and feedlot cattle from postweaning to slaughter.
TLDR
Findings from this study provide further evidence of non-O157 STEC shedding in beef cows and steer calves particularly at the stage of postweaning and before entry into the feedlot.
Comparison of Common Enrichment Broths Used in Diagnostic Laboratories for Shiga Toxin—Producing Escherichia coli
TLDR
Analysis of common broth media used for the enrichment of STEC suggests that diagnostic laboratories may benefit from enriching STEC samples in TSB as a first line enrichment instead of GN or Mac.
Detection of Shiga Toxin-Producing Escherichia coli from Nonhuman Sources and Strain Typing.
TLDR
Phenotypic assays and molecular tools for typing EHEC and STEC strains are used to detect and characterize human pathogenic strains among members of the STEC group.
Shiga toxin-producing Escherichia coli distribution and characterization in a pasture-based cow-calf production system.
TLDR
Repetitive element sequence-based PCR (rep-PCR) fingerprints were highly similar, supporting the hypothesis that strains are transmitted between animals but not necessarily from a dam to its calf, and suggesting that strain differences in transference may exist.
Development of a bacteriophage-based Method for Detection of Escherichia Coli O157:H7 in Fresh Vegetables
TLDR
A phage-based method using a recombinant phage for detection of E. coli O157:H7 in fresh vegetables was investigated, and the limit of detection was considered to be 2 CFU g-1 vegetable.
Microbead-based immunoassay for simultaneous detection of Shiga toxins and isolation of Escherichia coli O157 in foods.
TLDR
Using commercially available monoclonal antibodies coupled to carboxylated magnetic microbeads, an immunoassay capable of simultaneously serotyping E. coli O157 and detecting Stx (1) and/or Stx(2) is developed and indicates that Luminex technology has the potential to simultaneously detect multiple targets without loss of specificity and/ or sensitivity.
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