Growth and detachment of cell clusters from mature mixed-species biofilms.

@article{Stoodley2001GrowthAD,
  title={Growth and detachment of cell clusters from mature mixed-species biofilms.},
  author={Paul Stoodley and Steve Wilson and Luanne Hall-Stoodley and John D. Boyle and Hilary M. Lappin-Scott and John William Costerton},
  journal={Applied and environmental microbiology},
  year={2001},
  volume={67 12},
  pages={5608-13}
}
Detachment from biofilms is an important consideration in the dissemination of infection and the contamination of industrial systems but is the least-studied biofilm process. By using digital time-lapse microscopy and biofilm flow cells, we visualized localized growth and detachment of discrete cell clusters in mature mixed-species biofilms growing under steady conditions in turbulent flow in situ. The detaching biomass ranged from single cells to an aggregate with a diameter of approximately… CONTINUE READING