Growth and characterization of rabbit corneal cells in vitro

  title={Growth and characterization of rabbit corneal cells in vitro},
  author={STEPHEND. COOK and David A. Aitken and Sandra Marie Brown},
  journal={Graefe's Archive for Clinical and Experimental Ophthalmology},
Primary organ cultures of rabbit corneal epithelium, stroma, and endothelium were established by microdissection. Secondary cultures of epithelial cells, keratocytes, and endothelial cells were established by serial passage. The doubling time for epithelial cells and keratocytes was 18 h, and endothelial cells doubled their number in 5 days. Ultrastructural studies demonstrated characteristic morphological, nuclear, and cytoplasmic features of corneal epithelial cells, keratocytes, and… CONTINUE READING