Growth and Amino Acid Requirements of Various Strains of Group B Streptococci

  title={Growth and Amino Acid Requirements of Various Strains of Group B Streptococci},
  author={T. Milligan and T. Doran and D. Straus and S. Mattingly},
  journal={Journal of Clinical Microbiology},
  pages={28 - 33}
A chemically defined medium (FMC; B. Terleckyj, N. P. Willett, and G. D. Shockman, Infect. Immun. 11:649-655, 1975) was used to compare the growth and amino acid requirements of 16 strains of group B streptococci, consisting of both laboratory-passaged organisms and fresh clinical isolates from adult and neonatal infections. The 5 standard Lancefield immunizing strains of group B streptococci, 090 (Ia), H36B (Ib), A909 (Ic), 18RS21 (II), and D136C (III), had doubling times in FMC (28 to 36 min… Expand
In vitro method to differentiate isolates of type III Streptococcus agalactiae from symptomatic and asymptomatic patients
The results suggest that the physiological growth response of clinical isolates of group B streptococci to phosphate can serve as a diagnostic aid in screening potentially virulent strains in pregnant women and newborn infants. Expand
Growth of group E streptococci and production of antigens in a chemically defined medium.
  • G. Wessman
  • Biology, Medicine
  • Canadian journal of microbiology
  • 1982
Group E streptococci cells grown in CDM were less resistant to phagocytosis than those grown in the complex medium, but all strains produced antiphagocytic factor when cultured in either medium to which 10% porcine serum had been added. Expand
Extracellular products of type IIIStreptococcus agalactiae and their relationship to virulence
The high ETSA producers were shown to be significantly more virulent in mice than were the low producers, implying that the two different types of organisms have somewhat different mechanisms of pathogenicity in the mouse model. Expand
Kinetic and chemical analyses of the biologic significance of lipoteichoic acids in mediating adherence of serotype III group B streptococci
LTA appears to enhance the ability of virulent group B streptococci to bind to HEC and HFC with stronger avidity by virtue of the increased length of the cell-associated polymers synthesized by these strains. Expand
Association of elevated levels of extracellular neuraminidase with clinical isolates of type III group B streptococci
The results suggest that the ability to produce elevated levels of neuraminidase may be related to the frequent association of type III strains with disease among neonates. Expand
Association of type- and group-specific antigens with the cell wall of serotype III group B streptococcus
The covalent association of TSA, the group B polysaccharide, and the peptidoglycan was demonstrated by the presence of N-acetylmuramic acid, rhamnose, alanine, glutamate, and lysine in mutanolysin-extracted TSA material purified by DEAE-Sephacel anion exchange and Sepharose 4B gel chromatography. Expand
Temperature sensitivity of fructose-1,6-biphosphate aldolase accounts for the inability of the high-virulence clone ofStreptococcus agalactiae to grow at 40°C
The basis for the unusual growth inhibition at 40°C was examined in the present study and shown to be owing to a temperature-sensitive fructose-1,6-bisphosphate aldolase (fba). Expand
Comparative analysis of the localization of lipoteichoic acid in Streptococcus agalactiae and Streptococcus pyogenes
Results suggest that under normal growth conditions, the hydrophobic region (glycolipid) of LTA remains associated with the cytoplasmic membrane of GBS and unavailable forHydrophobic interactions at the cell surface with epithelial cells, in contrast, release of L TA into the environment by the GAS allows the fatty acid moieties to interact with hydrophilic domains on the surface of epithelial Cells. Expand
Lactic acid is a potential virulence factor for group B Streptococcus.
It is demonstrated that GBS-produced lactic acid is a potential virulence factor and may contribute to GBS invasive disease. Expand
Correlation between the production of extracellular substances by type III group B streptococcal strains and virulence in a mouse model
When levels of group B streptococci of each type were examined in organs of infected mice, comparable levels of organisms were found in the brain, spleen, and lungs of mice near death regardless of the initial inoculum, suggesting that these strains may be more invasive. Expand


Requirements for growth of Streptococcus agalactiae in a chemically defined medium
Despite extensive work on the group A streptococci (A. W. Bernheimer and A. M. Pappenheimer, Jr., J. Bacteriol. 43:481, 1942; H. D. Slade and W. C. Slamp, J. Exptl. Med. 102:291, 1955), very fewExpand
Amino acid requirements of Streptococcus mutans and other oral streptococci
The amino acid requirements of Streptococcus mutans strains AHT, OMZ-61, FA-1, BHT, GS-5, JC-2, Ingbritt, At6T, OMZ-176, 6715, Streptococcus salivarius HHT, Streptococcus sanguis OMZ-9, and strainExpand
Growth of several cariogenic strains of oral streptococci in a chemically defined medium
A chemically defined medium in which Streptococcus mutans strains AHT, BHT, GS-5, JC-2, Ingbritt, At6T, At9T, 6715, and OMZ-176 and StrePTococcus salivarius strain HHT grew rapidly to high turbidities was formulated and the growth rates of several of the strains tested were increased. Expand
Effects of nutritional characteristics of Streptococcus agalactiae on inhibition of growth by lactoperoxidase-thiocyanate-hydrogen peroxide in chemically defined culture medium
  • M. Mickelson
  • Medicine, Chemistry
  • Applied and environmental microbiology
  • 1976
Five cultures of Streptococcus agalactiae have an absolute requirement for L-cystine to grow in a chemically defined medium; hence, the growth requirement appears to be truly nutritional. Expand
Extracellular neuraminidase production by group B streptococci
Group B streptococcal neuraminidase was not active on human alpha-1 acid glycoprotein and did not show increased activity on bovine submaxillary mucin that had been O-deacetylated by alkaline treatment. Expand
Long-Chain Fatty Acid Inhibition of Growth of Streptococcus agalactiae in a Chemically Defined Medium
Antimicrobial activity of unsaturated and saturated fatty acids was reversed by bovine serum albumin and other compounds, suggesting a bacteriostatic action. Expand
Microcapsule of type III strains of group B Streptococcus: production and morphology
The yield of purified type III polysaccharide of group B Streptococcus was significantly improved by modification of the growth medium and a thicker microcapsule was found in organisms grown in standard broth. Expand
Immunological investigation of infants with septicemia or meningitis due to group B Streptococcus.
Sera from each of four adults with invasive infection who were studied during convalescence contained antibody to the capsular polysaccharide of type III group B Streptococcus, and antibody was absent from 10 infants who had recovered from bacteremia, septicemia, and/or meningitis due to type III Group B StrePTococcus. Expand
Transmission of group B streptococci among parturient women and their neonates.
Although race, ses, birth weight, and maternal obstetrical complications did not influence the prevalence of asymptomatic colonization with group B streptococci, low birth weight and prolonged rupture of membranes were significantly increased among neonates with proved infection of early onset type. Expand
Correlation of maternal antibody deficiency with susceptibility to neonatal group B streptococcal infection.
It is suggested that transplacental transfer of maternal antibody protects infants from invasive Group B streptococcal infection with Type III strains. Expand