Growth Inhibition of Mycoplasma by Inhibitors of Polyterpene Biosynthesis and Its Reversal by Cholesterol

@article{Smith1966GrowthIO,
  title={Growth Inhibition of Mycoplasma by Inhibitors of Polyterpene Biosynthesis and Its Reversal by Cholesterol},
  author={P. F. Smith and Carl V. Henrikson},
  journal={Journal of Bacteriology},
  year={1966},
  volume={91},
  pages={1854 - 1858}
}
Smith, Paul F. (University of South Dakota, Vermillion), and Carl V. Henrikson. Growth inhibition of Mycoplasma by inhibitors of polyterpene biosynthesis and its reversal by cholesterol. J. Bacteriol. 91:1854–1858. 1966.—Compounds which inhibit enzymatic reactions in the biosynthetic pathway to carotenoids inhibited growth of a sterol-nonrequiring species, Mycoplasma laidlawii, strain B, and M. hominis, strain 07. Since M. hominis lacks the enzymes for polyterpene biosynthesis, the inhibitory… 

Conversion of Mevalonic Acid to γ,γ-Dimethylallyl Pyrophosphate by Mycoplasma

Henrikson, Carl V. (University of South Dakota, Vermillion), and Paul F. Smith. Conversion of mevalonic acid to γ,γ-dimethylallyl pyrophosphate by Mycoplasma. J. Bacteriol. 92:701–706. 1966.—Three

Cholesterol Inhibition of Isopentenyl Pyrophosphate Δ3,Δ2-Isomerase in Mycoplasma laidlawii

Cholesterol inhibits isopentenyl Δ 3 ,Δ 2 -isomerase of Mycoplasma laidlawii in an apparently competitive fashion and no significant accumulation of phosphorylated intermediates of polyterpene biosynthesis was observed in cells or culture supernatant fluid.

The lipids of mycoplasma.

Characteristics of a New Sterol-nonrequiring Mycoplasma

Two Mycoplasma strains recovered from tissue culture environments were found to grow in complex media devoid of serum or serum fractions containing cholesterol and in a cholesterol-free synthetic medium, suggesting the need for some taxonomic changes in this group of organisms.

The structure and functions of the mycoplasma membrane.

  • D. Archer
  • Biology, Medicine
    International review of cytology
  • 1981

Lipoglycans from Mycoplasmas

Lipoglycans are associated with the cytoplasmic membranes of several genera of Mollicutes, namely Acholeplasma, Mycoplasma neurolyticum, Anaeroplasma , and Thermoplasma, and modulate the immune response to T-cell dependent antigens; they exhibit immunosuppressive and immunostimulatory activities.

Carotenoids acts as reinforcers of the Acholeplasma laidlawii lipid bilayer

The results support the notion that the carotenoids in A. laidlawii act as a rigid insert reinforcing the membrane bilayer, and show an increase in the mobility of the hydrocarbon chains of the spin-labeled fatty acids.

Role of sterols in membranes

It is interpreted to mean that the primary role sterols play in nature is a nonmetabolic one as architectural components of membranes and that this role can be played, but less well, by other molecules which approximate the steroidal structure.

References

SHOWING 1-10 OF 18 REFERENCES

Comparative Biosynthesis of Mevalonic Acid by Mycoplasma

The lack of beta-hydroxy-beta-methylglutaryl coenzyme A condensing enzyme and reductase activities in M. gallisepticum must occur after mevalonic acid in the biosynthetic pathway to terpenoids.

NONSAPONIFIABLE LIPIDS OF REPRESENTATIVE PLEUROPNEUMONIA-LIKE ORGANISMS

Cholesteryl-beta-d-glucoside was found to be formed by a fermentative, nonsterol-requiring strain only when supplied exogenous cholesterol, and no cholesterol or its derivatives were found in nonstersol-Requiring strains when grown in the absence of cholesterol.

THE CAROTENOID PIGMENTS OF MYCOPLASMA.

All the Mycoplasma organisms examined, which did not require a sterol as a nutrient, contained a carotenoid pigment identifiable as the hydrocarbon, neurosporene, and a carotinol, analogous to the sterol compounds found in sterol-requiring organisms.

Lysis of Mycoplasma, bacterial protoplasts, spheroplasts and L-forms by various agents.

The Mycoplasma and L-forms were much more resistant to lysis by osmotic shock and to alternate freezing and thawing than were the bacterial protoplasts and spheroplasts and the mycoplasmas were completely resistant to the lytic action of lysozyme.

EFFECTS OF ALPHA-P-CHLOROPHENOXYISOBUTYRYL ETHYL ESTER (CPIB) WITH AND WITHOUT ANDROSTERONE ON CHOLESTEROL BIOSYNTHESIS IN RAT LIVER.

CPIB is similar to dietary cholesterol in that it is not inhibitory when added to liver homogenates at concentrations up to about lop3 M, but in intact rats, both CPIB and Atromid significantly decreased the rate of cholesterol synthesis per gram of liver, as measured by the incorporation of acetate-l-C14 or of tritium water.

Quantitative measurement of the growth of pleuropneumonia-like organisms.

The purpose of this study was to develop and assess the suitability of media for cultivation of pleuropneumonia-like organisms by counting the number of colonies developing from 0.01 ml of liquid culture spread over the surface of an agar plate.

COMPARATIVE PHYSIOLOGY OF PLEUROPNEUMONIA-LIKE AND L-TYPE ORGANISMS.

A comparison study of the physical requirements for growth in relation to energy-Yielding and nonfermentative organisms found that energy-yielding organisms require more carbohydrates than nonfermentsative organisms.