Growth Characteristics and Enzyme Activity in Batrachochytrium dendrobatidis Isolates

@article{Symonds2008GrowthCA,
  title={Growth Characteristics and Enzyme Activity in Batrachochytrium dendrobatidis Isolates},
  author={Emma P Symonds and Darren J. Trott and Philip S. Bird and Paul C. Mills},
  journal={Mycopathologia},
  year={2008},
  volume={166},
  pages={143-147}
}
Batrachochytrium dendrobatidis is a member of the phylum Chytridiomycota and the causative organism chytridiomycosis, a disease of amphibians associated with global population declines and mass mortality events. The organism targets keratin-forming epithelium in adult and larval amphibians, which suggests that keratinolytic activity may be required to infect amphibian hosts. To investigate this hypothesis, we tested 10 isolates of B. dendrobatidis for their ability to grow on a range of keratin… 
View on Springer
ncbi.nlm.nih.gov
27 Citations
Highly Influential Citations
1
Background Citations
17
Methods Citations
2
Results Citations
1

27 Citations

Substrate-Specific Gene Expression in Batrachochytrium dendrobatidis, the Chytrid Pathogen of Amphibians

A genome-wide study of Bd gene expression under two different nutrient conditions and discusses the roles of these different genes as putative pathogenicity factors and what they can teach us about Bd’s metabolic targets, host invasion, and pathogenesis.

A novel subtilisin-like serine protease of Batrachochytrium dendrobatidis is induced by thyroid hormone and degrades antimicrobial peptides.

Batrachochytrium dendrobatidis: requirement for further isolate collection and archiving.

A summary of known Bd isolates that have been collected and archived in various locations around the world is provided and it is found that 53% originate from ranids in the United States.

Virulence and pathogenesis of chytridiomycosis: a lethal disease of amphibians

It is found that, after multiple passages, Bd is phenotypically plastic in its response to low nutrient concentrations, but may have an adaptive response to long-term maintenance in low temperatures, which could influence the host-pathogen dynamic in important ways.

Reptiles as potential vectors and hosts of the amphibian pathogen Batrachochytrium dendrobatidis in Panama.

This study is the first to provide evidence of non-amphibian carriers for B. dendrobatidis in a natural Neotropical environment and positively correlated with the infection prevalence among co-occurring anuran amphibians at any particular site.

History and recent progress on chytridiomycosis in amphibians

Chytrid fungus Batrachochytrium dendrobatidis has nonamphibian hosts and releases chemicals that cause pathology in the absence of infection

The results indicate that there are nonamphibian hosts for B. dendrobatidis and suggest that the fungus releases a chemical that can cause host pathology, even in the absence of infection, which might provide new hope for imperiled amphibians.

Batrachochytrium dendrobatidis zoospore secretions rapidly disturb intercellular junctions in frog skin.

Mosquitoes as a potential vector for the transmission of the amphibian chytrid fungus

This is the first study to demonstrate that an insect host may be a mechanical vector of Bd and suggests that the role of mosquitoes in the dissemination and control of the fungus should begin to consider.

Detection of pathogenic Batrachochytrium dendrobatidis using water filtration, animal and bait testing.

With continued refinement, semi-quantitative Bd water filtrate screening could better define zoospore-specific disease risk, allowing better characterization of the free-living phase of the organism's life cycle.

References

SHOWING 1-10 OF 18 REFERENCES

Physiology of Batrachochytrium dendrobatidis, a chytrid pathogen of amphibians.

The zoospore activity, physiology and protease production of B. dendrobatidis is investigated to help understand the epidemiology of this pathogen, which is implicated in severe population declines on several continents.

Cryo-archiving of Batrachochytrium dendrobatidis and other chytridiomycetes.

This work has adapted well-established techniques used for the long-term storage of tissue-culture cell lines to the preservation of B. dendrobatidis and other chytridiomycetes and has allowed these fungi to recover from storage at -80 degrees C and in liquid nitrogen over an extended period.

Strain differences in the amphibian chytrid Batrachochytrium dendrobatidis and non-permanent, sub-lethal effects of infection.

Disinfected frogs gained weight significantly more than infected frogs, to the point of becoming indistinguishable from controls, demonstrating that at least some of the effects of sub-lethal chytridiomycosis on hosts can be non-permanent and reversible.

Multilocus sequence typing suggests the chytrid pathogen of amphibians is a recently emerged clone

Only five variable nucleotide positions were detected among 10 loci, consistent with the description of B. dendrobatidis as a recently emerged disease agent, and electrophoretic karyotyping of multiple strains demonstrated a number of chromosome length polymorphisms.

Population genetics of the frog-killing fungus Batrachochytrium dendrobatidis

A population genetic comparison of Batrachochytrium dendrobatidis isolates from an intensively studied region of frog decline, the Sierra Nevada of California, finds low diversity, no amphibian-host specificity, little correlation between fungal genotype and geography, local frog extirpation by a single fungalgenotype, and evidence of human-assisted fungus migration.

Similarities and Specificities of Fungal Keratinolytic Proteases: Comparison of Keratinases of Paecilomyces marquandii and Doratomyces microsporus to Some Known Proteases

Both keratinases of Paecilomyces marquandii and Doratomyces microsporus possess broad cleavage specificity with a preference for aromatic and nonpolar amino acid residues at the P-1 position, and were significantly more active on keratin than subtilisin, trypsin, elastase, chymotrypsIn, or collagenase.

Differentiation of Penicillium griseofulvum Dierckx isolates by enzyme assays and by patulin and griseofulvin analyses

The production of patulin and griseofulvin by 49 different isolates of Penicillium griseofulvum Dierckx was analyzed by high-performance liquid chromatography. Eleven isolates were obtained from…

Ultrastructural and molecular phylogenetic delineation of a new order, the Rhizophydiales (Chytridiomycota).

Feather keratin hydrolysis by a Vibrio sp. strain kr2

Strain kr2 shows potential for use for biotechnological processes involving keratin hydrolysis, presenting similarities with that reported for feather lysate, feather meal and raw feathers.

Analysis of the enzymatic activity of mycelial and yeast phases of Penicillium marneffei.

There was some inter-isolate variation in both mycelia and yeast in the activities of other enzymes such as esterases and galactosidases, but enzymes did not change significantly over the course of culturing in three representative isolates.