Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications

@article{Sato2013GrowingSM,
  title={Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications},
  author={Toshiro Sato and Hans Clevers},
  journal={Science},
  year={2013},
  volume={340},
  pages={1190 - 1194}
}
Recent examples have highlighted how stem cells have the capability to initiate morphogenesis in vitro; that is, to generate complex structures in culture that closely parallel their in vivo counterparts. Lgr5, the receptor for the Wnt-agonistic R-spondins, marks stem cells in multiple adult organs of mice and humans. In R-spondin–based three-dimensional cultures, these Lgr5 stem cells can grow into ever-expanding epithelial organoids that retain their original organ identity. Single Lgr5 stem… 

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