Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications

@article{Sato2013GrowingSM,
  title={Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications},
  author={Toshiro Sato and Hans Clevers},
  journal={Science},
  year={2013},
  volume={340},
  pages={1190 - 1194}
}
Recent examples have highlighted how stem cells have the capability to initiate morphogenesis in vitro; that is, to generate complex structures in culture that closely parallel their in vivo counterparts. Lgr5, the receptor for the Wnt-agonistic R-spondins, marks stem cells in multiple adult organs of mice and humans. In R-spondin–based three-dimensional cultures, these Lgr5 stem cells can grow into ever-expanding epithelial organoids that retain their original organ identity. Single Lgr5 stem… 
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Methods Citations
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908 Citations

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Current knowledge on the multiform reconstitution of intestinal stem cell niche

The recent advances in the microfabrication of intestinal organoids on hydrogel systems are reviewed to contribute to multiform reconstitution of the ISC niche.
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