Group-specific differentiation between high- and low-risk human papillomavirus genotypes by general primer-mediated PCR and two cocktails of oligonucleotide probes.

@article{Jacobs1995GroupspecificDB,
  title={Group-specific differentiation between high- and low-risk human papillomavirus genotypes by general primer-mediated PCR and two cocktails of oligonucleotide probes.},
  author={Marcel V Jacobs and Ana Maria de Roda Husman and Adriaan J. C. van den Brule and Peter J. F. Snijders and Chris J L M Meijer and Jan M. M. Walboomers},
  journal={Journal of clinical microbiology},
  year={1995},
  volume={33 4},
  pages={901-5}
}
In recent years, general primer-mediated PCR assays have been developed to detect a broad spectrum of human papillomavirus (HPV) genotypes. In this study, a procedure enabling a simple group-specific differentiation of high-risk (HPV-16, -18, -31, -33, -35, -39, -45, -51, -52, -54, -56, and -58) and low risk (HPV-6, -11, -34, -40, -42, -43, and 44) HPVs following an HPV general primer-mediated (GP5+/GP6+) PCR is presented. By computer-assisted sequence analysis, oligonucleotides (30-mers… CONTINUE READING

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