Green marker for colonies of Bacillus subtilis.

@article{Itaya2001GreenMF,
  title={Green marker for colonies of Bacillus subtilis.},
  author={Mitsuhiro Itaya and Syed Mohammed Shaheduzzaman and Kenji Matsui and Akane Omori and Tadao Tsuji},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2001},
  volume={65 3},
  pages={
          579-83
        }
}
A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell… CONTINUE READING
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