Green fluorescent protein labeling of primordial germ cells using a nontransgenic method and its application for germ cell transplantation in salmonidae.

@article{Yoshizaki2005GreenFP,
  title={Green fluorescent protein labeling of primordial germ cells using a nontransgenic method and its application for germ cell transplantation in salmonidae.},
  author={Goro Yoshizaki and Yasuko Tago and Yutaka Takeuchi and Etsuko Sawatari and Terumasa Kobayashi and Toshio Takeuchi},
  journal={Biology of reproduction},
  year={2005},
  volume={73 1},
  pages={88-93}
}
Transplanting primordial germ cells (PGCs) has a number of potential applications in fish bioengineering. Previously, we established a system to visualize live PGCs in the rainbow trout by introducing the green fluorescent protein (Gfp) gene driven by rainbow trout vasa gene regulatory regions. However, for PGC transplantation to be practically useful in aquaculture, visualization of PGCs using a nontransgenic technique is required. In this study, we demonstrate a method for labeling PGCs from… CONTINUE READING

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Primordial germ-cell development: the zebrafish perspective

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