Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells.

@article{Soboleski2005GreenFP,
  title={Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells.},
  author={Mark R. Soboleski and Jason Oaks and William P. Halford},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={2005},
  volume={19 3},
  pages={440-2}
}
Green fluorescent protein (GFP) has gained widespread use as a tool to visualize spatial and temporal patterns of gene expression in vivo. However, it is not generally accepted that GFP can also be used as a quantitative reporter of gene expression. We report that GFP is a reliable reporter of gene expression in individual eukaryotic cells when fluorescence is measured by flow cytometry. Two pieces of evidence support this conclusion: GFP fluorescence increases in direct proportion to the GFP… CONTINUE READING

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