Green fluorescent protein expressed by recombinant pseudorabies virus as an in vivo marker for viral replication.

@article{Jns1997GreenFP,
  title={Green fluorescent protein expressed by recombinant pseudorabies virus as an in vivo marker for viral replication.},
  author={A. J{\"o}ns and Thomas C Mettenleiter},
  journal={Journal of virological methods},
  year={1997},
  volume={66 2},
  pages={283-92}
}
We isolated and characterized a pseudorabies virus (PrV) mutant expressing an engineered green fluorescent protein (GFP) optimized for expression in human cells. The GFP DNA was inserted in the non-essential glycoprotein G (gG) gene of the attenuated PrV strain Bartha. The coding sequence was cloned in frame behind the first seven codons of the gG gene under control of the strong gG promotor. On excitation with blue light, live cells infected with the recombinant PrV B80eGFP exhibited bright… CONTINUE READING