Green fluorescent protein as a visual selection marker for papaya (Carica papaya L.) transformation

@article{Zhu2004GreenFP,
  title={Green fluorescent protein as a visual selection marker for papaya (Carica papaya L.) transformation},
  author={Y. J. Zhu and R. Agbayani and P. Moore},
  journal={Plant Cell Reports},
  year={2004},
  volume={22},
  pages={660-667}
}
Chemical-based selection for plant transformation is associated with a number of real and perceived problems that might be avoided through visual selection. We have used green fluorescent protein (GFP), as a visual selectable marker to produce transformed papaya (Carica papaya) plants following microprojectile bombardment of embryogenic callus. GFP selection reduced the selection time from 3 months on a geneticin (G418) antibiotic-containing medium to 3–4 weeks. Moreover, GFP selection… Expand
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