Green fluorescent protein as a noninvasive stress probe in resting Escherichia coli cells.

@article{Cha1999GreenFP,
  title={Green fluorescent protein as a noninvasive stress probe in resting Escherichia coli cells.},
  author={Hyung Joon Cha and R Srivastava and Vikram N. Vakharia and Govind Rao and William E. Bentley},
  journal={Applied and environmental microbiology},
  year={1999},
  volume={65 2},
  pages={409-14}
}
We constructed and characterized three stress probe plasmids which utilize a green fluorescent protein as a noninvasive reporter in order to elucidate Escherichia coli cellular stress responses in quiescent or resting cells. Cellular stress levels were easily detected by fusing three heat shock stress protein promoter elements, those of the heat shock transcription factor sigma32, the protease subunit ClpB, and the chaperone DnaK, to the reporter gene gfpuv. When perturbed by a chemical or… CONTINUE READING

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