Green fluorescent protein as a noninvasive intracellular pH indicator.

  title={Green fluorescent protein as a noninvasive intracellular pH indicator.},
  author={Malea Kneen and Javier Farinas and Yun Li and Alan S. Verkman},
  journal={Biophysical journal},
  volume={74 3},
It was found that the absorbance and fluorescence of green fluorescent protein (GFP) mutants are strongly pH dependent in aqueous solutions and intracellular compartments in living cells. pH titrations of purified recombinant GFP mutants indicated >10-fold reversible changes in absorbance and fluorescence with pKa values of 6.0 (GFP-F64L/S65T), 5.9 (S65T), 6.1 (Y66H), and 4.8 (T203I) with apparent Hill coefficients of 0.7 for Y66H and approximately 1 for the other proteins. For GFP-S65T in… CONTINUE READING
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