Green-fluorescent protein as a new vital marker in plant cells.

  title={Green-fluorescent protein as a new vital marker in plant cells.},
  author={Jen Sheen and S Y Hwang and Yasuo Niwa and Hiroko Kobayashi and D. W. Galbraith},
  journal={The Plant journal : for cell and molecular biology},
  volume={8 5},
The green-fluorescent protein (GFP) from jellyfish Aequorea victoria has been used as a convenient new vital marker in various heterologous systems. However, it has been problematic to express GFP in higher eukaryotes, especially in higher plants. This paper reports that either a strong constitutive or a heat-shock promoter can direct the expression of GFP which is easily detectable in maize mesophyll protoplasts. In this single-cell system, bright green fluorescence emitted from GFP is visible… CONTINUE READING
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