Green fluorescent protein as a marker for Pseudomonas spp.

  title={Green fluorescent protein as a marker for Pseudomonas spp.},
  author={Guido Vincent Bloemberg and George A O'Toole and Ben Lugtenberg and Roberto Kolter},
  journal={Applied and environmental microbiology},
  volume={63 11},
The development of sensitive methods for observing individual bacterial cells in a population in experimental models and natural environments, such as in biofilms or on plant roots, is of great importance for studying these systems. We report the construction of plasmids which constitutively express a bright mutant of the green fluorescent protein of the jellyfish Aequorea victoria and are stably maintained in Pseudomonas spp. We demonstrate the utility of these plasmids to detect individual… CONTINUE READING

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