Green fluorescent protein as a marker for gene expression.

  title={Green fluorescent protein as a marker for gene expression.},
  author={Martin Chalfie and Y Tu and Ghia M. Euskirchen and William W. Ward and Douglas C. Prasher},
  volume={263 5148},
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms. 

Green Fluorescent Protein as a Reporter To Monitor Gene Expression and Food Colonization by Aspergillus flavus

ABSTRACT Transformants of Aspergillus flavus containing theAequorea victoria gfp gene fused to a viral promoter or the promoter region and 483 bp of the coding region of A. flavus aflR expressed

Red Fluorescent Protein (DsRed) as a Reporter inSaccharomyces cerevisiae

The utilization of a red fluorescent protein (DsRed) as an in vivo marker for Saccharomyces cerevisiae is described and a series of vectors are now available which can be used to create amino-terminal and carboxyl- terminal fusions with the DsRed protein.

Fluorescent protein spectra.

The cloning of the green fluorescent protein (GFP) from the jellyfish Aequoria victoria and its expression in heterologous systems was a significant advance for optical microscopy of living cells

Improved green fluorescent protein as a fast reporter of gene expression in plant cells

An improved green fluorescent protein (GFP), S65TGFP, has new properties that make itself more suitable as a reporter of gene expression. The coding sequence for S65TGFP was placed under the control

A codon-optimized green fluorescent protein for live cell imaging in Zymoseptoria tritici☆

Green Fluorescent Protein Green Fluorescent Protein

GFP is a protein in the jellyfish Aequorea Victoria that exhibits green fluorescence when exposed to light and is used to study cells in embryos and fetuses during developmental processes.

Green Fluorescent Protein Is Lighting Up Fungal Biology

Prasher cloned a cDNA for the green fluorescent protein (GFP) gene from the jellyfish Aequorea victoria in 1992 and shortly thereafter this gene or derivatives thereof were successfully expressed and conferred fluorescence to bacteria and yeast.



Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein.

The characterization of the Aequorea victoria GFP chromophore is described, which is released as a hexapeptide upon digestion of the protein with papain, formed upon cyclization of the residues Ser-dehydroTyr-Gly within the polypeptide.

The genetics of Caenorhabditis elegans.

Estimates of the induced mutation frequency of both the visible mutants and X chromosome lethals suggests that, just as in Drosophila, the genetic units in C. elegans are large.

Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.

A dominant behavioral marker, rol‐6(su‐1006), and an efficient microinjection procedure which facilitate the recovery of Caenorhabditis elegans transformants are described and it is shown that low copy number extrachromosomal transformation can be achieved by adjusting the relative concentration of DNA molecules in the injection mixture.

lux genes and the applications of bacterial bioluminescence.

The phenomenon of bacterial bioluminescence can now be captured and applied within any bacterial species from several rather different perspectives and provides a real-time noninvasive reporter for measuring gene expression, a sensitive marker for bacterial detection and a measure of intracellular biochemical function, i.e. as a holistic determinant of cellular viability.

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.

Intermolecular energy transfer in the bioluminescent system of Aequorea.

In the present study, GFP has been purified, crystallized, and partially characterized and an energy transfer in citro from aequorin to this protein has been demonstrated.