Green fluorescent protein as a marker for gene expression.

@article{Chalfie1994GreenFP,
  title={Green fluorescent protein as a marker for gene expression.},
  author={Martin Chalfie and Y Tu and Ghia M. Euskirchen and William W. Ward and Douglas C. Prasher},
  journal={Science},
  year={1994},
  volume={263 5148},
  pages={
          802-5
        }
}
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms. 
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References

SHOWING 1-10 OF 25 REFERENCES
Firefly luciferase as a tool in molecular and cell biology.
Primary structure of the Aequorea victoria green-fluorescent protein.
A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans.
Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein.
TLDR
The characterization of the Aequorea victoria GFP chromophore is described, which is released as a hexapeptide upon digestion of the protein with papain, formed upon cyclization of the residues Ser-dehydroTyr-Gly within the polypeptide.
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
The genetics of Caenorhabditis elegans.
TLDR
Estimates of the induced mutation frequency of both the visible mutants and X chromosome lethals suggests that, just as in Drosophila, the genetic units in C. elegans are large.
Structure of the chromophore of Aequorea green fluorescent protein
Vectors for selective expression of cloned DNAs by T7 RNA polymerase.
Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.
TLDR
A dominant behavioral marker, rol‐6(su‐1006), and an efficient microinjection procedure which facilitate the recovery of Caenorhabditis elegans transformants are described and it is shown that low copy number extrachromosomal transformation can be achieved by adjusting the relative concentration of DNA molecules in the injection mixture.
lux genes and the applications of bacterial bioluminescence.
TLDR
The phenomenon of bacterial bioluminescence can now be captured and applied within any bacterial species from several rather different perspectives and provides a real-time noninvasive reporter for measuring gene expression, a sensitive marker for bacterial detection and a measure of intracellular biochemical function, i.e. as a holistic determinant of cellular viability.
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