Green fluorescent protein as a marker for gene expression.
@article{Chalfie1994GreenFP, title={Green fluorescent protein as a marker for gene expression.}, author={Martin Chalfie and Y Tu and Ghia M. Euskirchen and William W. Ward and Douglas C. Prasher}, journal={Science}, year={1994}, volume={263 5148}, pages={ 802-5 } }
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.
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GFP is a protein in the jellyfish Aequorea Victoria that exhibits green fluorescence when exposed to light and is used to study cells in embryos and fetuses during developmental processes.
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