• DOI:10.1126/SCIENCE.8303295
Corpus ID: 9043327

Green fluorescent protein as a marker for gene expression.

@article{Chalfie1994GreenFP,
  title={Green fluorescent protein as a marker for gene expression.},
  author={M. Chalfie and Y. Tu and G. Euskirchen and W. Ward and D. Prasher},
  journal={Science},
  year={1994},
  volume={263 5148},
  pages={
          802-5
        }
}

M. Chalfie, Y. Tu, +2 authors D. Prasher

Published 1994

Biology, Chemistry, Medicine

Science

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A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.