Green fluorescent protein (GFP)-dependent separation of bacterial ghosts from intact cells by FACS.

@article{Haidinger2001GreenFP,
  title={Green fluorescent protein (GFP)-dependent separation of bacterial ghosts from intact cells by FACS.},
  author={Wolfgang Haidinger and Michael P. Szostak and Wolfgang Beisker and Werner Lubitz},
  journal={Cytometry},
  year={2001},
  volume={44 2},
  pages={106-12}
}
BACKGROUND E. coli and Salmonella ghost preparations, produced by applying the PhiX174 protein E-mediated lysis system, contain nonlysed bacteria at a very low percentage. To use the ghosts as vaccines, additional methods have to be identified to remove any viable cell, to end up in totally inactivated ghost fractions. Materials and Methods To increase the purity of ghost fractions, we established a green fluorescent protein (GFP)-dependent "in vivo staining" method to be combined with the E… CONTINUE READING