Green fluorescent antibodies: novel in vitro tools.

@article{Casey2000GreenFA,
  title={Green fluorescent antibodies: novel in vitro tools.},
  author={Jennifer L. Casey and Alex Brandon Coley and Leann Tilley and Michael Foley},
  journal={Protein engineering},
  year={2000},
  volume={13 6},
  pages={
          445-52
        }
}
We produced a fluorescent antibody as a single recombinant protein in Escherichia coli by fusing a red-shifted mutant of green fluorescent protein (EGFP) to a single-chain antibody variable fragment (scFv) specific for hepatitis B surface antigen (HepBsAg). GFP is a cytoplasmic protein and it was not previously known whether it would fold correctly to form a fluorescent protein in the periplasmic space of E.COLI: In this study we showed that EGFP alone or fused to the N'- and C'-termini of the… CONTINUE READING
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