Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction.

Abstract

We attempted to test the idea that the relative abundance of each individual tRNA isoacceptor in Escherichia coli can be altered by varying its cognate codon concentration. In order to change the overall codon composition of the messenger pool, we have expressed in E. coli lacZ with the aid of T7 RNA polymerase so that their respective gene products… (More)

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@article{Dong1995GratuitousOO, title={Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction.}, author={Hualin Dong and Lina Nilsson and Charles G. Kurland}, journal={Journal of bacteriology}, year={1995}, volume={177 6}, pages={1497-504} }