Gratuitous induction by N-acetylmannosamine of germ tube formation and enzymes for N-acetylglucosamine utilization in Candida albicans

  title={Gratuitous induction by N-acetylmannosamine of germ tube formation and enzymes for N-acetylglucosamine utilization in Candida albicans},
  author={P. Sullivan and M. Shepherd},
  journal={Journal of Bacteriology},
  pages={1118 - 1122}
N-Acetylmannosamine did not support the growth of Candida albicans, and this sugar was not accumulated by cells. Incubation of starved yeast cells at 37 degrees C with N-acetylmannosamine plus glucose resulted in germ tube formation. Furthermore, N-acetylmannosamine alone induced the uptake system for N-acetylglucosamine and the enzymes of the N-acetylglucosamine catabolic pathway to the same extent as the natural substrate. Induction of the uptake system and the enzymes was observed at 28… Expand
An analysis of the metabolism and cell wall composition of Candida albicans during germ-tube formation.
Uptake of N-acetylglucosamine and glutamine was inhibited by cycloheximide which suggests that de novo protein synthesis was required for the induction of these uptake systems. Expand
Candida albicans germ-tube formation with immobilized GlcNAc
Germ-tube formation by Candida albicans is the initial phase of the transition from yeast growth by budding to mycelial growth by hyphal elongation and branching. Conditions which influence germtubeExpand
Antibiotic tetaine — a selective inhibitor of chitin and mannoprotein biosynthesis in Candida albicans
The antibiotic tetaine inhibits in Candida albicans the biosynthesis of two important cell wall constituents, chitin and mannoprotein, and in the presence of tetaine, probably a modified mannobrotein, lacking a branched polymannan, is synthesized. Expand
A Comparison of Germ Tube Production by Candida albicans in Three Media
Medium 199 and Glucose-Glycine Medium at 37˚C were highly satisfactory and gave 90 % conversion of yeast cells to hyphal-form cells and appeared to give more rapid germ tube production than 199 Medium. Expand
Hyphal development in Candida albicans from different cell states
Current understanding of the molecular mechanism underlying hyphal development, the best studied virulence factor in C. albicans, is described, which will expand the number of potential drug targets with novel modes of action for anti-virulence therapeutics. Expand
Effect of calcium ion uptake on Candida albicans morphology.
It is concluded that unrestricted calcium uptake resulted in the specific inhibition of C. albicans mycelial growth, indicating a critical role for calcium in the regulation of C.'s morphogenesis. Expand
Biochemical and pharmacological factors causing induction and suppression of germination of Trichosporon beigelii.
Germination was suppressed by high concentrations of glucose, increasing inocula, low pH, and amphotericin B at achievable serum concentrations, and many of the factors regulating germination of T. beigelii appear to be similar to those for Candida albicans. Expand
N-acetylglucosamine sensing by a GCN5-related N-acetyltransferase induces transcription via chromatin histone acetylation in fungi
This work has identified a gene that is essential for GlcNAc signalling (NGS1) in Candida albicans, a commensal and pathogenic yeast of humans and conserved in diverse fungi that have Glc NAc catabolic genes. Expand
Microbial β-N-acetylhexosaminidases and their biotechnological applications
This survey compares amino acid sequence data, biochemical characteristics, and catalytic properties of β-N-acetylhexosaminidases from a number of microbial sources. Expand
Cell envelope of Candida albicans.
  • M. Shepherd
  • Chemistry, Medicine
  • Critical reviews in microbiology
  • 1987
The cell envelope of the human pathogenic yeast Candida albicans includes the plasma membrane and the mannoproteins, enzymes, beta-glucans, and chitin of the wall, which contains the antigenic determinants of the yeast cells. Expand


Glucose repression of the inducible catabolic pathway for N-acetylglucosamine in yeast.
  • B. Singh, A. Datta
  • Biology, Medicine
  • Biochemical and biophysical research communications
  • 1978
It is concluded that “glucose effect” or catabolite repression is absent in Candida albicans, a pathogenic strain of yeast. Expand
Induction of N-acetylmannosamine catabolic pathway in yeast.
Experiments with inhibitors of macromolecule synthesis suggest that the snythesis of RNA and protein is necessary for the induction of these cyzymes whereas the synthesis of DNA is not. Expand
Yeast-mycelial conversion induced by N-acetyl-D-glucosamine in Candida albicans
There is chemical, cytochemical and ultrastructural evidence that the differences between Y and M walls of C. albicans are essentially quantitative, strongly suggesting that hyphal conversion in this yeast is controlled by the modulation of pre-existing enzymatic activities rather than by any new factors. Expand
Chitin Synthesis in Candida albicans: Comparison of Yeast and Hyphal Forms
Chitin synthesis was studied in both yeast and hyphae of the dimorphic fungus Candida albicans, and enzyme activity was found to be located on the inner side of the plasma membrane. Expand
Induction of N-acetylglucosamine kinase in yeast.
Experiments with RNA- and protein-synthesis inhibitors indicate that the appearance of new enzyme activity is dependent on concomitant new protein synthesis and the inducer operates at a transcriptional level. Expand
Germ tube induction in Candida albicans.
A reproducible and simple system for the production of germ tubes from yeast cells of Candida albicans using glucose and glutamine as substrates has been described and although the DNA content did not change for the first 4 h of germ tube formation, the RNA content more than doubled. Expand
Inducibility of germ-tube formation in Candida albicans at different phases of yeast growth.
Summary: Yeast cells of Candida albicans growing at 25 °C in glucose/yeast extract medium cannot germinate by dilution into fresh medium at 37 °C before reaching the stationary phase of growth.Expand
Ultrastructural investigations of the formation of Candida albicans germ tubes and septa.
Both germ tube and blastospore formation begin with comparable changes in the cell wall of the mother cell, and pseudomycelia can be clearly discerned by means of transmission electron microscopy. Expand
Ultrastructural changes in the wall during germ-tube formation from blastospores of Candida albicans.
The wall of a blastospore of Candida albicans is organized in a multilayer structure with amorphous, granular and fibrous components of various electron densities, and some data suggested that the electron-transparent layer that developed during germ-tube formation was rich in chitin. Expand
Candida and candidosis
A comprehensive and critical review of the medical and scientific literature on Candida infections by a leading authority in the field. Covers all aspects of the subject, including epidemiology,Expand