Gratuitous induction by N-acetylmannosamine of germ tube formation and enzymes for N-acetylglucosamine utilization in Candida albicans

@article{Sullivan1982GratuitousIB,
  title={Gratuitous induction by N-acetylmannosamine of germ tube formation and enzymes for N-acetylglucosamine utilization in Candida albicans},
  author={P. Sullivan and M. Shepherd},
  journal={Journal of Bacteriology},
  year={1982},
  volume={151},
  pages={1118 - 1122}
}
N-Acetylmannosamine did not support the growth of Candida albicans, and this sugar was not accumulated by cells. Incubation of starved yeast cells at 37 degrees C with N-acetylmannosamine plus glucose resulted in germ tube formation. Furthermore, N-acetylmannosamine alone induced the uptake system for N-acetylglucosamine and the enzymes of the N-acetylglucosamine catabolic pathway to the same extent as the natural substrate. Induction of the uptake system and the enzymes was observed at 28… Expand
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References

SHOWING 1-10 OF 16 REFERENCES
Glucose repression of the inducible catabolic pathway for N-acetylglucosamine in yeast.
  • B. Singh, A. Datta
  • Biology, Medicine
  • Biochemical and biophysical research communications
  • 1978
TLDR
It is concluded that “glucose effect” or catabolite repression is absent in Candida albicans, a pathogenic strain of yeast. Expand
Induction of N-acetylmannosamine catabolic pathway in yeast.
TLDR
Experiments with inhibitors of macromolecule synthesis suggest that the snythesis of RNA and protein is necessary for the induction of these cyzymes whereas the synthesis of DNA is not. Expand
Yeast-mycelial conversion induced by N-acetyl-D-glucosamine in Candida albicans
TLDR
There is chemical, cytochemical and ultrastructural evidence that the differences between Y and M walls of C. albicans are essentially quantitative, strongly suggesting that hyphal conversion in this yeast is controlled by the modulation of pre-existing enzymatic activities rather than by any new factors. Expand
Chitin Synthesis in Candida albicans: Comparison of Yeast and Hyphal Forms
TLDR
Chitin synthesis was studied in both yeast and hyphae of the dimorphic fungus Candida albicans, and enzyme activity was found to be located on the inner side of the plasma membrane. Expand
Induction of N-acetylglucosamine kinase in yeast.
TLDR
Experiments with RNA- and protein-synthesis inhibitors indicate that the appearance of new enzyme activity is dependent on concomitant new protein synthesis and the inducer operates at a transcriptional level. Expand
Germ tube induction in Candida albicans.
TLDR
A reproducible and simple system for the production of germ tubes from yeast cells of Candida albicans using glucose and glutamine as substrates has been described and although the DNA content did not change for the first 4 h of germ tube formation, the RNA content more than doubled. Expand
Inducibility of germ-tube formation in Candida albicans at different phases of yeast growth.
Summary: Yeast cells of Candida albicans growing at 25 °C in glucose/yeast extract medium cannot germinate by dilution into fresh medium at 37 °C before reaching the stationary phase of growth.Expand
Ultrastructural investigations of the formation of Candida albicans germ tubes and septa.
TLDR
Both germ tube and blastospore formation begin with comparable changes in the cell wall of the mother cell, and pseudomycelia can be clearly discerned by means of transmission electron microscopy. Expand
Ultrastructural changes in the wall during germ-tube formation from blastospores of Candida albicans.
TLDR
The wall of a blastospore of Candida albicans is organized in a multilayer structure with amorphous, granular and fibrous components of various electron densities, and some data suggested that the electron-transparent layer that developed during germ-tube formation was rich in chitin. Expand
Candida and candidosis
A comprehensive and critical review of the medical and scientific literature on Candida infections by a leading authority in the field. Covers all aspects of the subject, including epidemiology,Expand
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