Grating imager systems for fluorescence optical-sectioning microscopy.

Abstract

In fluorescence microscopy, optical sectioning is defined as the attenuation or removal of out-of-focus features from an image, and it is a prerequisite for quantitative analysis of three-dimensional structure or function within the specimen. Optical sectioning is most commonly performed by confocal scanning fluorescence microscopy or two-photon scanning… (More)
DOI: 10.1101/pdb.top083493

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