Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing.

  title={Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing.},
  author={Huahua Yue and Xiaoming Zhou and Meng Cheng and Da Xing},
  volume={10 3},
Direct cellular delivery of CRISPR/Cas9 complexes is of great significance for genome editing and other recently developed applications, such as gene expression regulation and RNA/DNA imaging. Here, we first constructed a graphene oxide (GO)-polyethylene glycol (PEG)-polyethylenimine (PEI) nanocarrier for the delivery of high-molecular-weight Cas9/single-guide RNA (sgRNA) complexes for endocytosis, endosomal escape, nuclear entry, and gene editing. The results demonstrate that the nanocarrier… CONTINUE READING
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