Granulocytes without degranulation: neutrophil function in granule-depleted cytoplasts.

  title={Granulocytes without degranulation: neutrophil function in granule-depleted cytoplasts.},
  author={Helen M. Korchak and Dirk Roos and Kenneth N. Giedd and E M Wynkoop and Katalin Vienne and L E Rutherford and J P Buyon and Abby M. Rich and Gerald Weissmann},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={80 16},
Neutrophils respond to a variety of stimuli by generating superoxide anion, degranulating, and aggregating. Because it has been suggested that fusion of granules with the plasmalemma (degranulation) is necessary for aggregation and superoxide anion generation, we have tested whether these responses can be demonstrated in "neutrophilic cytoplasts" (granule-free vesicles of cytoplasm enclosed by plasmalemma). When examined by electron microscopy, cytoplasts were found to be approximately 4… CONTINUE READING