Granulocyte-macrophage colony stimulating factor from human lymphocytes. The effect of glycosylation on receptor binding and biological activity.

@article{Cebon1990GranulocytemacrophageCS,
  title={Granulocyte-macrophage colony stimulating factor from human lymphocytes. The effect of glycosylation on receptor binding and biological activity.},
  author={Jonathon Cebon and Nicos A. Nicola and Marty Ward and Iain Gardner and P J Dempsey and Judith E. Layton and Ullrich D{\"u}hrsen and Antony W. Burgess and Ed Nice and George Morstyn},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 8},
  pages={4483-91}
}
Native human granulocyte-macrophage colony stimulating factor (hGM-CSF) has previously been purified using methods which typically required several sequential chromatographic steps and only yielded small amounts of hGM-CSF. We have purified and characterized hGM-CSF using monoclonal antibodies raised against bacterially synthesized hGM-CSF. Activated donor T-lymphocytes grown in interleukin-2 and then reactivated with phytohemagglutinin produce several forms of hGM-CSF which can be purified… CONTINUE READING

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