Granulocyte colony-stimulating factor induction of normal human bone marrow progenitors results in neutrophil-specific gene expression.

@article{Berliner1995GranulocyteCF,
  title={Granulocyte colony-stimulating factor induction of normal human bone marrow progenitors results in neutrophil-specific gene expression.},
  author={Nancy Berliner and Ann B Hsing and Timothy A. Graubert and F Sigurdsson and Mona Zain and Edward Bruno and Ronald F. Hoffman},
  journal={Blood},
  year={1995},
  volume={85 3},
  pages={799-803}
}
We have used a combination of hematopoietic growth factors to induce in vitro granulocytic maturation. A fraction of marrow cells enriched for hematopoietic progenitor cells (CD34+, HLA-DR+) was isolated from normal human bone marrow by monoclonal antibody staining and fluorescence-activated cell sorting. Cells were cultured in a suspension system for 3 days in the presence of stem cell factor and interleukin-3 (IL-3), after which granulocyte colony-stimulating factor (G-CSF) was added. Cells… CONTINUE READING