Granulocyte-colony stimulating factor and stem cell factor are the crucial factors in long-term culture of human primitive hematopoietic cells supported by a murine stromal cell line.

@article{Nishi1996GranulocytecolonySF,
  title={Granulocyte-colony stimulating factor and stem cell factor are the crucial factors in long-term culture of human primitive hematopoietic cells supported by a murine stromal cell line.},
  author={Nozomu Nishi and Rika Ishikawa and Hiroyuki Inoue and Mitsuo Nishikawa and Minoru Kakeda and Takashi Yoneya and Hiroto Tsumura and Haruhiko Ohashi and Yusuke Yamaguchi and Kazuhiro Motoki and Tetsuo Sudo and Kazuhiro John Mori},
  journal={Experimental hematology},
  year={1996},
  volume={24 11},
  pages={1312-21}
}
The findings that murine marrow stromal cell line MS-5 supported the proliferation of human lineage-negative (Lin-) CD34+CD38- bone marrow cells in long-term culture have been reported. In this study, we analyzed this proliferating activity of MS-5-conditioned medium (CM) on human primitive hematopoietic cells. When Lin-CD34+CD38- cells of normal human cord blood cells were co-cultured with MS-5, colony forming cells (CFCs) were maintained over 7 weeks in vitro. Prevention of contact between MS… CONTINUE READING

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