Gramicidin S-synthetase. Preparation of the multienzymic complex with a high specific activity.

@article{Koischwitz1976GramicidinSP,
  title={Gramicidin S-synthetase. Preparation of the multienzymic complex with a high specific activity.},
  author={H. Koischwitz and Horst Kleinkauf},
  journal={Biochimica et biophysica acta},
  year={1976},
  volume={429 3},
  pages={1041-51}
}
A new purification procedure for the multienzyme of gramicidin S-synthetase has been developed. In vitro proteolysis with partial inactivation is suppressed by protease inhibitors EDTA, phenylmethylsulfonylfluoride, and fast preparation methods during initial separation steps. Activity has only been assayed by the total reaction of gramicidin S-synthetase, not by partial reactions of amino acid activation. The assay has been improved by evaluation of inhibitory concentrations of buffers, salts… CONTINUE READING