Gramicidin S-synthetase. Electrophoretic characterization of the multienzyme.

  title={Gramicidin S-synthetase. Electrophoretic characterization of the multienzyme.},
  author={H. Koischwitz and Horst Kleinkauf},
  journal={Biochimica et biophysica acta},
  volume={429 3},
1. A method characterizing the fully active gramicidin S-synthetase (EC. 6.3.2.-) multienzyme in protein mixtures by a combination of sedimentation and polyacrylamide gel electrophoretic mobility data has been described. 2. The molecular weight of 280000 has been reevaluated by gradient centrifugation, gel filtration, and polyacrylamide gel electrophoresis in presence of sodium dodecyl sulfate. The size of the multienzyme is not changed by sodium dodecyl sulfate treatment. 3. In polyacrylamide… CONTINUE READING