Gram-scale synthesis of uridine 5'-diphospho-N-acetylglucosamine: comparison of enzymic and chemical routes

@article{Heidlas1992GramscaleSO,
  title={Gram-scale synthesis of uridine 5'-diphospho-N-acetylglucosamine: comparison of enzymic and chemical routes},
  author={Jurgen E. Heidlas and Watson J. Lees and Patrick Pale and George M. Whitesides},
  journal={Journal of Organic Chemistry},
  year={1992},
  volume={57},
  pages={146-151}
}
practical chemoenzymatic and chemical routes to uridine 5'-diphospho-N'acetylglucosamine (UDP-GlcNAc) on r !ruscale have been developed. The chemoenzymatic synthesis provided convenient access to glucosaminigpnosphate and N-acetylgluiosamine&phosphatc (GlcNAc-GP) in )lGmmol quantities. The condensation betweenblcNAc-6-P and UTi, was catalyzed by readily available crude enzyme extracts from dried cells of the ieit Co"aida utitis and afforde d a LTVo yield of UDP-GlcNAc from GlcNAc-6-P. The… Expand
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