Grafting of protein L-binding activity onto recombinant antibody fragments.

  title={Grafting of protein L-binding activity onto recombinant antibody fragments.},
  author={Julien Muzard and Sonia Adi-Bessalem and Matthieu O. Juste and Laraba-Djebari Fatima and Nicolas Aubrey and Philippe Billiald},
  journal={Analytical biochemistry},
  volume={388 2},
Recombinant antibody fragments consisting of variable domains can be easily produced in various host cells, but there is no universal system that can be used to purify and detect them in the free form or complexed with their antigen. Protein L (PpL) is a cell wall protein isolated from Peptostreptococcus magnus, which has been reported to interact with the V-KAPPA chain of some, but not all, antibodies. Here we grafted the V-KAPPA framework region 1 (FR1) sequence of a high-affinity PpL-binding… CONTINUE READING