Graded activation of CRAC channel by binding of different numbers of STIM1 to Orai1 subunits

@article{Li2011GradedAO,
  title={Graded activation of CRAC channel by binding of different numbers of STIM1 to Orai1 subunits},
  author={Zhengzheng Li and Lin Liu and Yongqiang Deng and Wei Ji and Wenzhong Du and Pingyong Xu and Liangyi Chen and Tao Xu},
  journal={Cell Research},
  year={2011},
  volume={21},
  pages={305-315}
}
The Ca2+ release-activated Ca2+ (CRAC) channel pore is formed by Orai1 and gated by STIM1 after intracellular Ca2+ store depletion. To resolve how many STIM1 molecules are required to open a CRAC channel, we fused different numbers of Orai1 subunits with functional two-tandem cytoplasmic domains of STIM1 (residues 336-485, designated as S domain). Whole-cell patch clamp recordings of these chimeric molecules revealed that CRAC current reached maximum at a stoichiometry of four Orai1 and eight S… CONTINUE READING
BETA

From This Paper

Figures, tables, and topics from this paper.

Explore Further: Topics Discussed in This Paper

Citations

Publications citing this paper.
SHOWING 1-10 OF 68 CITATIONS, ESTIMATED 52% COVERAGE

130 Citations

01020'11'13'15'17'19
Citations per Year
Semantic Scholar estimates that this publication has 130 citations based on the available data.

See our FAQ for additional information.

References

Publications referenced by this paper.
SHOWING 1-10 OF 36 REFERENCES

STIM 1 gates the storeoperated calcium channel ORAI 1 in vitro

  • Y Zhou, P Meraner, HT Kwon
  • Nat Struct Mol Biol
  • 2010

Similar Papers

Loading similar papers…