GraFix: stabilization of fragile macromolecular complexes for single particle cryo-EM.

@article{Stark2010GraFixSO,
  title={GraFix: stabilization of fragile macromolecular complexes for single particle cryo-EM.},
  author={Holger Stark},
  journal={Methods in enzymology},
  year={2010},
  volume={481},
  pages={109-26}
}
Here, we review the GraFix (Gradient Fixation) method to purify and stabilize macromolecular complexes for single particle cryo-electron microscopy (cryo-EM). During GraFix, macromolecules undergo a weak, intramolecular chemical cross-linking while being purified by density gradient ultracentrifugation. GraFix-stabilized particles can be used directly for… CONTINUE READING