Golgi staining by two fluorescent ceramide analogues in cultured fibroblasts requires metabolism.

@article{Ptz1995GolgiSB,
  title={Golgi staining by two fluorescent ceramide analogues in cultured fibroblasts requires metabolism.},
  author={Uwe P{\"u}tz and G{\"u}nter Schwarzmann},
  journal={European journal of cell biology},
  year={1995},
  volume={68 2},
  pages={
          113-21
        }
}
A fluorescent derivative of ceramide, N-(6-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-aminohexanoyl)-D-erythr o-sphingosine (C6-NBD-Cer), has been shown to label the Golgi apparatus of cultured cells (Lipsky, N. G., R. E. Pagano, Science 228, 745-747 (1985)). There is no unequivocal explanation for this Golgi labeling which is important in view of photolabeling of Golgi proteins which might be involved in metabolism, sorting and transport of (glyco)sphingolipids. To gain more insight into the mode… CONTINUE READING

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