Golden Gate cloning.

  title={Golden Gate cloning.},
  author={Carola Engler and Sylvestre Marillonnet},
  journal={Methods in molecular biology},
DNA assembly methods are essential tools for biological research and biotechnology. Therefore various methods have been developed to clone DNA fragments of interest. Conventional methods usually require several cloning steps to generate a construct of interest. At each step, a single DNA fragment is transferred from a donor plasmid or PCR product to a recipient vector. In the past few years, a number of methods have been developed to facilitate and speed up this process. One of these methods… CONTINUE READING

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