Gold nanoparticle-mediated (GNOME) laser perforation: a new method for a high-throughput analysis of gap junction intercellular coupling

@article{Begandt2015GoldN,
  title={Gold nanoparticle-mediated (GNOME) laser perforation: a new method for a high-throughput analysis of gap junction intercellular coupling},
  author={Daniela Begandt and Augustinus Bader and Georgios C. Antonopoulos and Markus Schomaker and Stefan Kalies and Heiko Meyer and Tammo Ripken and Anaclet Ngezahayo},
  journal={Journal of Bioenergetics and Biomembranes},
  year={2015},
  volume={47},
  pages={441-449}
}
The present report evaluates the advantages of using the gold nanoparticle-mediated laser perforation (GNOME LP) technique as a computer-controlled cell optoperforation to introduce Lucifer yellow (LY) into cells in order to analyze the gap junction coupling in cell monolayers. To permeabilize GM-7373 endothelial cells grown in a 24 multiwell plate with GNOME LP, a laser beam of 88 μm in diameter was applied in the presence of gold nanoparticles and LY. After 10 min to allow dye uptake and… CONTINUE READING
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