Gold-facilitated in situ hybridization: a bright-field autometallographic alternative to fluorescence in situ hybridization for detection of Her-2/neu gene amplification.

@article{Tubbs2002GoldfacilitatedIS,
  title={Gold-facilitated in situ hybridization: a bright-field autometallographic alternative to fluorescence in situ hybridization for detection of Her-2/neu gene amplification.},
  author={Raymond R. Tubbs and James D. Pettay and Marek Skacel and Richard Douglas Powell and Mark H. Stoler and Patrick C. Roche and James F. Hainfeld},
  journal={The American journal of pathology},
  year={2002},
  volume={160 5},
  pages={1589-95}
}
Fluorescence in situ hybridization (FISH) represents an excellent method for profiling gene amplification in situ, but correlation with tissue morphology is difficult because of dark-field visualization. Validation of a bright-field assay for assessment of HER-2/neu gene amplification was investigated. Streptavidin-Nanogold was used to generate bright-field gene copy signals using GoldEnhance gold-based autometallography, catalyzed reported deposition, and a biotin-labeled probe. One hundred… CONTINUE READING