Glycosylphosphatidylinositol-specific phospholipase D of human serum--activity modulation by naturally occurring amphiphiles.

Abstract

The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substrate. The enzyme is stimulated by n… (More)

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@article{Rhode2000Glycosylphosphatidylinositolspecific, title={Glycosylphosphatidylinositol-specific phospholipase D of human serum--activity modulation by naturally occurring amphiphiles.}, author={Holly K Rhode and Matthias B Schulze and Gerhard A Cumme and Angela G{\"o}hlert and Elizabeth D. Blume and Ralf Bublitz and K. Schilling and A. Horn}, journal={Biological chemistry}, year={2000}, volume={381 5-6}, pages={471-85} }