Glycosylation steps during spiramycin biosynthesis in Streptomyces ambofaciens: involvement of three glycosyltransferases and their interplay with two auxiliary proteins.

@article{Nguyen2010GlycosylationSD,
  title={Glycosylation steps during spiramycin biosynthesis in Streptomyces ambofaciens: involvement of three glycosyltransferases and their interplay with two auxiliary proteins.},
  author={Hoang Chuong Nguyen and Fatma Karray and Sylvie Lautru and Josette Gagnat and Ahmed Lebrihi and Thuy Duong Ho Huynh and Jean-Luc Pernodet},
  journal={Antimicrobial agents and chemotherapy},
  year={2010},
  volume={54 7},
  pages={2830-9}
}
Streptomyces ambofaciens synthesizes spiramycin, a 16-membered macrolide antibiotic used in human medicine. The spiramycin molecule consists of a polyketide lactone ring (platenolide) synthesized by a type I polyketide synthase, to which three deoxyhexoses (mycaminose, forosamine, and mycarose) are attached successively in this order. These sugars are essential to the antibacterial activity of spiramycin. We previously identified four genes in the spiramycin biosynthetic gene cluster predicted… CONTINUE READING