Glycosylation of human cyclooxygenase-2 (COX-2) decreases the efficacy of certain COX-2 inhibitors.

@article{Sevigny2012GlycosylationOH,
  title={Glycosylation of human cyclooxygenase-2 (COX-2) decreases the efficacy of certain COX-2 inhibitors.},
  author={Mary B Sevigny and Kamara Graham and Esmeralda Ponce and Maggie C. Louie and Kylie J Mitchell},
  journal={Pharmacological research},
  year={2012},
  volume={65 4},
  pages={445-50}
}
Prostanoids play an important role in a variety of physiological and pathophysiological processes including inflammation and cancer. The rate-limiting step in the prostanoid biosynthesis pathway is catalyzed by cyclooxygenase-2 (COX-2). COX-2 exists as two glycoforms, 72 and 74 kDa, the latter resulting from an additional glycosylation at Asn(580). In this study, Asn(580) was mutated, and the mutant and wild-type COX-2 genes were expressed in COS-1 cells to determine how glycosylation affects… CONTINUE READING