Glycosylation of flavonoids with a glycosyltransferase from Bacillus cereus.

  title={Glycosylation of flavonoids with a glycosyltransferase from Bacillus cereus.},
  author={Jae Hyung Ko and Bong Gyu Kim and Ahn Joong-Hoon},
  journal={FEMS microbiology letters},
  volume={258 2},
Microbial glycosyltransferases can convert many small lipophilic compounds such as phenolics, terpenoids, cyanohydrins and alkaloids into glycons using uridine-diphosphate-activated sugars. The main chemical functions of glycosylation processes are stabilization, detoxification and solubilization of the substrates. The gene encoding the UDP-glycosyltransferase from Bacillus cereus, BcGT-1, was cloned by PCR and sequenced. BcGT-1 was expressed in Escherichia coli BL21 (DE3) with a his-tag and… CONTINUE READING
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