Glycosylation catalyzed by lysyl hydroxylase 3 is essential for basement membranes

@article{Ruotsalainen2006GlycosylationCB,
  title={Glycosylation catalyzed by lysyl hydroxylase 3 is essential for basement membranes},
  author={Heli Ruotsalainen and Laura Sipil{\"a} and Miia H. Vapola and Raija T. Sormunen and Antti M. Salo and Lahja Uitto and Derry K. Mercer and Simon P. Robins and Maija Risteli and Attila Asz{\'o}di and Reinhard F{\"a}ssler and Raili Myllylä},
  journal={Journal of Cell Science},
  year={2006},
  volume={119},
  pages={625 - 635}
}
Lysyl hydroxylase 3 (LH3) is a multifunctional enzyme possessing lysyl hydroxylase (LH), hydroxylysyl galactosyltransferase (GT) and galactosylhydroxylysyl glucosyltransferase (GGT) activities in vitro. To investigate the in vivo importance of LH3-catalyzed lysine hydroxylation and hydroxylysine-linked glycosylations, three different LH3-manipulated mouse lines were generated. Mice with a mutation that blocked only the LH activity of LH3 developed normally, but showed defects in the structure… 

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