Glycosylation Influences Voltage-Dependent Gating of Cardiac and Skeletal Muscle Sodium Channels

@article{Zhang1999GlycosylationIV,
  title={Glycosylation Influences Voltage-Dependent Gating of Cardiac and Skeletal Muscle Sodium Channels},
  author={Yi Zhang and Hali A. Hartmann and Jonathan Satin},
  journal={The Journal of Membrane Biology},
  year={1999},
  volume={171},
  pages={195-207}
}
The role of glycosylation on voltage-dependent channel gating for the cloned human cardiac sodium channel (hH1a) and the adult rat skeletal muscle isoform (μl) was investigated in HEK293 cells transiently transfected with either hH1a or μl cDNA. The contribution of sugar residues to channel gating was examined in transfected cells pretreated with various glycosidase and enzyme inhibitors to deglycosylate channel proteins. Pretreating transfected cells with enzyme inhibitors castanospermine and… CONTINUE READING