Glycosyl modification facilitates homo- and hetero-oligomerization of the serotonin transporter. A specific role for sialic acid residues.

@article{Ozaslan2003GlycosylMF,
  title={Glycosyl modification facilitates homo- and hetero-oligomerization of the serotonin transporter. A specific role for sialic acid residues.},
  author={Deniz Ozaslan and Sophie Yu Wang and Billow A. Ahmed and Arif M Kocabas and John C McCastlain and Anca Bene and Fusun Kilic},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 45},
  pages={43991-4000}
}
The serotonin transporter (SERT) is an oligomeric glycoprotein with two sialic acid residues on each of two complex oligosaccharide molecules. In this study, we investigated the contribution of N-glycosyl modification to the structure and function of SERT in two model systems: wild-type SERT expressed in sialic acid-defective Lec4 Chinese hamster ovary (CHO) cells and a mutant form (after site-directed mutagenesis of Asn-208 and Asn-217 to Gln) of SERT, QQ, expressed in parental CHO cells. In… CONTINUE READING