Glycosaminoglycans promote HARP/PTN dimerization.

  title={Glycosaminoglycans promote HARP/PTN dimerization.},
  author={Isabelle Bernard-Pierrot and M{\'e}lanie H{\'e}roult and Gilles Lemaitre and Denis Barritault and J. M. Courty and Pierre Emmanuel Milhiet},
  journal={Biochemical and biophysical research communications},
  volume={266 2},
Heparin affin regulatory peptide (HARP), also called pleiotrophin (PTN), is a secreted polypeptide which binds to heparin and plays a key role in cellular growth and differentiation. In order to assess the determinants potentially important to its biological activity, we tested the ability of HARP to oligomerize, a process involved in mitogenic activity of the heparin-binding fibroblast growth factor. Using dissuccinimidyl suberate cross-linking experiments and affinity chromatography, we… CONTINUE READING