Glycosaminoglycans bind factor Xa in a Ca2+-dependent fashion and modulate its catalytic activity.

@article{OBrien2003GlycosaminoglycansBF,
  title={Glycosaminoglycans bind factor Xa in a Ca2+-dependent fashion and modulate its catalytic activity.},
  author={Lee A O'Brien and Alan R. Stafford and James C Fredenburgh and Jeffrey I Weitz},
  journal={Biochemistry},
  year={2003},
  volume={42 44},
  pages={13091-8}
}
Recent studies have demonstrated the existence of a Ca(2+)-dependent heparin-binding site on factor Xa. To characterize this heparin-binding site, the extrinsic fluorescence of fluorescein-labeled, active site-blocked factor Xa was monitored as it was titrated with glycosaminoglycans of various sulfate content and chain length. The binding of glycosaminoglycans to factor Xa appears to be charge-dependent because affinity is correlated with degree of glycosaminoglycan sulfation. All… CONTINUE READING